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Part:BBa_K4574020
11aa-APG-Linker
APG-repeat based short linker designed for seamless fusion of immunoglobulins to single-chain variable fragments (scFv). It possesses the proper length and flexibility to optimise the functionality of the connected domains.
Source
The following basic part was developed for scFv fusion to an antibody by Hultqvist et al.[1].
The provided sequence was derived through back-translation from the resulting peptide. This back-translation took into account the codon usage bias for expression in mammalian cells (see "Usage and Biology" section). Additionally, the sequence was designed to comply with the BioBrick assembly standards.
Usage and Biology
This basic part has been used as a component of several antibody constructs in mammalian cell culture (HEK293T, Exi293, RAMOS).
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
References
- ↑ Rofo, F., Meier, S. R., Metzendorf, N. G., Morrison, J. I., Petrovic, A., Syvänen, S., Sehlin, D., & Hultqvist, G. (2022). A Brain-Targeting Bispecific-Multivalent Antibody Clears Soluble Amyloid-Beta Aggregates in Alzheimer’s Disease Mice. Neurotherapeutics, 19(5), 1588–1602. https://doi.org/10.1007/s13311-022-01283-y
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